Introduction: Methotrexate (MTX) could provoke a renal dysfunction. However, beneficial
extra-hematopoietic effect of erythropoietin might guard against MTX-induced nephrotoxicity.
Objectives: Determination of renoprotective erythropoietin’s role against MTX-induced
nephrotoxicity through elucidating its renofunctional and renomorphological effects in adult
male albino rats.
Materials and Methods: The study was performed on 60 adult male Albino rats, equally
divided into three groups; group 1 (control): treated with intraperitoneal injections of normal
saline at a dosage of 0.5 mg/kg BW twice weekly for 9 weeks. group 2: injected with MTX
hydrate intraperitoneal twice weekly at a dosage of 0.5 mg/kg BW for 9 weeks; and group
3: intraperitoneal injected with MTX hydrate in a similar dosage and duration like group 2
concomitant with subcutaneous injection of 100 IU/kg recombinant human erythropoietin
once weekly for 9 weeks. At the study end, serum urea and creatinine together with albuminuria
were measured, rats were sacrificed and renal sections were prepared for histopathological
Results: Significantly increased values of renal function analyzed substances with deteriorated
histopathological renal changes were detected in the MTX-treated group compared to
either the control or to the MTX and erythropoietin co-treated group. The later displayed
statistically significant decreased levels of the substances accompanied by remarkably
ameliorated microscopic renal changes. Additionally, insignificant statistical biochemical and
morphological renal differences were noticed between the third and control groups.
Conclusion: This study concluded valuable and efficient defense against MTX-induced
nephrotoxicity in adult male Albino rats when co-treated with erythropoietin.