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Submitted: 09 Jun 2020
Accepted: 10 Aug 2019
ePublished: 06 Sep 2019
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J Nephropharmacol. 2020;9(2): e15.
doi: 10.34172/npj.2020.15

Scopus ID: 85085869820
  Abstract View: 216
  PDF Download: 92

Original

Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats

Majid Tavafi 1 * ORCID logo, Hassan Ahmadvand 2, Ahmad Tamjidipour 1, Afshin Hasanvand 3 ORCID logo

1 Department of Anatomy, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
*Corresponding author: Prof. Majid Tavafi, Ph.D, Email: mtavafi@yahoo.com and tavafi.m@lums.ac.ir

Abstract

Introduction: Reactive oxygen species (ROS) are the main factors in pathogenesis of renal ischemia-reperfusion (I/R) injury.

Objectives: The aim of this study was to evaluate the renoprotective effect of rosmarinic acid (ROA) as an antioxidant substance against renal I/R injury.

Materials and Methods: Forty male rats were divided into five groups. Group 1 control; group 2 ischemia reperfusion (I/R). Groups 3, 4 and 5 I/R treated by ROA 50, 100 and 200 mg/kg respectively. The treated groups (groups 3, 4 and 5) received ROA one hour before ischemia induction. Renal ischemia was induced by ligating of renal vessels through vascular clips. After 45 minutes of ischemia, the clips were removed to make renal recirculation (reperfusion). Twenty-four hours after the onset of reperfusion, under anesthesia blood were sampled and kidneys were removed. The serum and supernatant of renal homogenate were prepared. Serum creatinine, nitric oxide (NO) and paraoxonase (PON) were measured. The concentration of renal malondialdehyde (MDA), glutathione peroxidase (GPX), glutathione (GSH) and catalase (CAT) activity were assessed.

Results: The administration of ROA, decreased serum creatinine and increased serum NO and PON compared to group 2 (P<0.05). Renal MDA, GSH, GPX and CAT activity improved significantly in animals that received ROA in comparison to group 2.

Conclusion: Administration of ROA improved renal I/R injuries via inhibition of lipid peroxidation and increase of GSH, GPX, CAT, NO and PON.

Keywords: Oxidative stress, Kidney, Antioxidant, Ischemia-reperfusion, Rosmarinic acid, Reactive oxygen species

Implication for health policy/practice/research/medical education:

Decrease of renal ATP during ischemia and specially increase of reactive oxygen species (ROS) during renal reperfusion are the main reasons for renal injury following ischemia and reperfusion (I/R). In this study, rosmarinic acid (ROA) was administered to improve renal I/R injury. ROA attenuated renal I/R injuries through inhibition of lipid peroxidation and increase of renal glutathione (GSH), glutathione peroxidase (GPX), catalase (CAT), paraoxonase (PON) and nitric oxide (NO).

Please cite this paper as: Tavafi M, Ahmadvand H, Tamjidipour A, Hasanvand A. Rosmarinic acid ameliorates renal ischemia reperfusion damage in rats. J Nephropharmacol. 2020;9(2):e15. DOI: 10.34172/npj.2020.15.

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